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Biotransformation of MiPT to 4-HO-MiPT Options
 
benzyme
#1 Posted : 5/23/2020 10:59:28 PM

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after much contemplation, I finally got around to prepping PFtek jars with 15 mM MiPT (made from tryptamine, derived from tryptophan) as an experimental group, and a control group without MiPT. Characterization will be by HPLC-UV-MS.

https://www.reddit.com/r...ansformation_of_mipt_to/
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dragonrider
#2 Posted : 5/23/2020 11:20:20 PM

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Would this work as well with organisms that produce bufotenin?
 
downwardsfromzero
#3 Posted : 5/23/2020 11:26:24 PM

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dragonrider wrote:
Would this work as well with organisms that produce bufotenin?

Maybe not. The 5-hydroxylation of tryptophan occurs before decarboxylation in that pathway, I think. Not much is known about the isopropyl analogue of bufotenine.
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DeDao
#4 Posted : 5/25/2020 3:26:16 PM

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This is one of the coolest things I had ever heard and I've glad you are bringing it to light once more. Very peculiar and interesting...
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benzyme
#5 Posted : 5/25/2020 3:33:16 PM

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Big grin

Thanks for the support. It's been discussed before, I'm just fortunate of having the means of characterizing the end products. LCMS determination of psilocin/psilocybin in mushrooms is something I'm no stranger to, and I have spectra for the MiPT.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
 
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