CHATPRIVACYDONATELOGINREGISTER
DMT-Nexus
FAQWIKIHEALTH & SAFETYARTATTITUDEACTIVE TOPICS
Biotransformation of MiPT to 4-HO-MiPT Options
 
benzyme
#1 Posted : 5/23/2020 10:59:28 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7287
Joined: 21-May-2008
Last visit: 14-Jul-2021
Location: the lab
after much contemplation, I finally got around to prepping PFtek jars with 15 mM MiPT (made from tryptamine, derived from tryptophan) as an experimental group, and a control group without MiPT. Characterization will be by HPLC-UV-MS.

https://www.reddit.com/r...ansformation_of_mipt_to/
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 

Trippy glass for trippy people.
 
dragonrider
#2 Posted : 5/23/2020 11:20:20 PM

DMT-Nexus member

Moderator

Posts: 2317
Joined: 09-Jul-2016
Last visit: 25-Jul-2021
Location: spacetime
Would this work as well with organisms that produce bufotenin?
 
downwardsfromzero
#3 Posted : 5/23/2020 11:26:24 PM

Peeing into the abyss

Chemical expertSenior Member

Posts: 5895
Joined: 30-Aug-2008
Last visit: 29-Jul-2021
Location: square root of minus one
dragonrider wrote:
Would this work as well with organisms that produce bufotenin?

Maybe not. The 5-hydroxylation of tryptophan occurs before decarboxylation in that pathway, I think. Not much is known about the isopropyl analogue of bufotenine.
Ora, lege, lege, lege, relege et labora

“There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work."
― Jacques Bergier, quoting Fulcanelli
 
benzyme
#4 Posted : 5/25/2020 3:33:16 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7287
Joined: 21-May-2008
Last visit: 14-Jul-2021
Location: the lab
Big grin

Thanks for the support. It's been discussed before, I'm just fortunate of having the means of characterizing the end products. LCMS determination of psilocin/psilocybin in mushrooms is something I'm no stranger to, and I have spectra for the MiPT.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#5 Posted : 8/15/2020 7:27:56 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7287
Joined: 21-May-2008
Last visit: 14-Jul-2021
Location: the lab
welp, those spores were nonviable. I just innoc'd some jars with a new syringe (most vendors have opted for syringes vs. prints), with 20 mM MiPT, and a control jar.

I also ordered a syringe, and a print, of c.cyanescens. That species may particularly have stronger expression of PsiH, a gene which encodes for tryptamine-4-monooxygenase (a CYP450 enzyme, nonspecific oxidoreductase). Said gene was only recently characterized, by Fricke et. al, 2017.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#6 Posted : 12/6/2020 5:38:02 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7287
Joined: 21-May-2008
Last visit: 14-Jul-2021
Location: the lab
Confused
benzyme attached the following image(s):
control.jpg (343kb) downloaded 56 time(s).
experimental.JPG (204kb) downloaded 54 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
RoundAbout
#7 Posted : 12/6/2020 10:00:03 PM

DMT-Nexus member


Posts: 283
Joined: 19-Nov-2018
Last visit: 29-Jul-2021
I guess the main peak in the control is a fragment from the alpha cleavage of psilocybin, but what are the main peaks in the experimental? Out of curiousity...
 
benzyme
#8 Posted : 12/7/2020 12:08:27 AM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7287
Joined: 21-May-2008
Last visit: 14-Jul-2021
Location: the lab
RoundAbout wrote:
I guess the main peak in the control is a fragment from the alpha cleavage of psilocybin, but what are the main peaks in the experimental? Out of curiousity...


your guess is as good as mine. I didn't run any standards
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
 
Users browsing this forum
Guest

DMT-Nexus theme created by The Traveler
This page was generated in 0.015 seconds.