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benzyme
#1 Posted : 9/12/2018 2:20:32 AM

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From what I've seen, there aren't many close up photos (besides microscopic) on the webs. I just collected these specimens, not even an hour ago. The close-up middle of the top photo is newly formed sclerotia.
Still early in the season, they typically form hardened brown/black brain-like sclerotia when the weather gets cold in late fall/early winter.
benzyme attached the following image(s):
IMG_5305.jpg (2,175kb) downloaded 391 time(s).
IMG_5304.jpg (2,661kb) downloaded 388 time(s).
IMG_5309.jpg (2,945kb) downloaded 383 time(s).
IMG_5307.jpg (2,128kb) downloaded 384 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 

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dragonrider
#2 Posted : 9/12/2018 11:05:37 AM

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Are you gonna make yourself some kykeon benz?

But seriously, how is it that all naturally occurring lysergamides come from fungi that grow on the seeds of plants? Is claviceps related to the fungi on morning glory and hawaian baby woodrose, or is it just something in the biochemistry of fungi in general that makes fungi produce lysergamides, but not plants or algea?
 
benzyme
#3 Posted : 9/12/2018 12:54:07 PM

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first, I’ll plate honeydew; and get some pics of the organism under the [compound] microscope.
Then sterilize the sclerotia in 15% saline, and cut slerotia, then plate those. after a couple weeks, I’d do a couple transfers, and do characterization under UV, and some preliminary Van urk’s reagent tests. if they check out, onto fermentation of secondary metabolites.

I don’t know the relation to endophytic fungi found in HBWR and MG; they may be distant cousins. their mechanism is the same: hijack tryptophan, and use it in the DMATS pathway. Humidity and temp tend to influence the levels of alk production of the fungus on live plants.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
downwardsfromzero
#4 Posted : 9/12/2018 3:29:00 PM

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dragonrider wrote:
how is it that all naturally occurring lysergamides come from fungi that grow on the seeds of plants?

Footnote here: Some races of Lolium arundinaceum syn. Festuca arundinacea (tall fescue) contain ergoline alkaloids, principally ergovaline, in the leaves. I haven't found anything about the alkaloid content of its seeds yet. Epichloë coenophiala is the endophyte responsible for alkaloid production in this instance. (Published yields would suggest that tall fescue is not a viable source species for ergoline alkaloids.)

benzyme wrote:
Humidity and temp tend to influence the levels of alk production of the fungus on live plants.

Me being lazy and all, do you have a good reference for some specific data on this?

Great project, looking forward to further reports Thumbs up
Ora, lege, lege, lege, relege et labora

“There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work."
― Jacques Bergier, quoting Fulcanelli
 
benzyme
#5 Posted : 9/12/2018 4:32:16 PM

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http://b-ok.xyz/book/510008/a93655


"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#6 Posted : 9/13/2018 3:42:23 AM

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ahh, I missed the part where they are referring to rye.

anywho, if you find any interesting info specific to paspali in that book, please post. I plan to generate a crap-ton of data.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
downwardsfromzero
#7 Posted : 9/13/2018 2:57:47 PM

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Thanks for the link.

I'll continue with my general-purpose data scouring and will be sure to post any relevant comments brought up.
Ora, lege, lege, lege, relege et labora

“There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work."
― Jacques Bergier, quoting Fulcanelli
 
dragonrider
#8 Posted : 9/14/2018 8:09:45 PM

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It is a while ago now, but on a couple of occasions i have taken 1P-LSD in conjunction with some morning glory seeds.

The very odd thing was...when the seeds where old, the effects of the 1P-LSD where dulled, but with fresh seeds the effects where being amplified...significantly. It went as far as having OBE's, wich i never had with LSD by itself.

It may seem as if the degradation of LSH into LSA, is responsible for this. But i wonder if there may be other substances in these seeds, and probably the claviceps species as well, that could explain the very extreme synergistic effects i experienced.

Are there lysergamides, that modulate receptors that LSD is active on?
 
benzyme
#9 Posted : 9/14/2018 8:32:10 PM

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in short, yes..with varying degrees of agonism/antagonism. Ergoline pharmacology is rather complex, given the variety of structures and their different binding affinities on the various 5HT-subtypes
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#10 Posted : 9/14/2018 9:51:26 PM

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Cool
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#11 Posted : 9/14/2018 10:23:15 PM

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This is the academic (ATCC) strain, from the original line I worked with in 2011. I kept it in the freezer since then.
Just plated this last week, on plain-jane PDA. I prepped more PDA plates today, with chloramphenicol, for transfers.

benzyme attached the following image(s):
IMG_5318.jpg (2,434kb) downloaded 294 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#12 Posted : 9/16/2018 1:33:13 AM

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http://b-ok.xyz/book/2264805/fffb0b
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7DC50E81-47F7-4A63-B59D-335E5CBB8F46.png (913kb) downloaded 280 time(s).
3997A1B2-1023-4E12-9B5B-CF745181A216.png (1,239kb) downloaded 279 time(s).
7037329D-5987-436B-B1D2-598E429A64F4.png (1,724kb) downloaded 276 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
dragonrider
#13 Posted : 9/16/2018 12:04:04 PM

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benzyme wrote:
in short, yes..with varying degrees of agonism/antagonism. Ergoline pharmacology is rather complex, given the variety of structures and their different binding affinities on the various 5HT-subtypes

That is very interesting. I was planning to do some more experiments with combining lysergamides, but the times it DID work, the effects where so extreme and unpredictable that it frightened me a little.

But maybe i should not let that put me off. If i can get my hands on some fresh seeds again, wich shouldn't be too difficult.....

If we would know more about how this works exactly, wich chemicals in wich quantities, etc, the consequences could be significant. It's like adding a whole new dimension to the LSD experience.
 
benzyme
#14 Posted : 9/16/2018 2:59:20 PM

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check the link above those pages I uploaded, your question was what prompted me to link that book . It is pharmacologically comprehensive.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
dragonrider
#15 Posted : 9/16/2018 4:56:38 PM

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OK thanks.
 
benzyme
#16 Posted : 9/22/2018 3:34:39 AM

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here's a better pic of a new sclerotial cell. collected it from a trail/pond in my neighborhood. there's another underneath, in the middle.
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"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#17 Posted : 9/23/2018 8:17:40 PM

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major score today. lots of sclerotia.
plated some honeydew on chloramphenicol-PDA, after my last attempts ended up with bacterial colonies.

benzyme attached the following image(s):
IMG_5412.jpg (1,773kb) downloaded 217 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#18 Posted : 9/26/2018 11:22:10 PM

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after three days, this is what I got. Purple sectors are high-producers, from what I've read. the plate is clean, with chloramphenicol, it better be.
the dark spots are just carbon deposits, from the flame-sterilized tweezers.
benzyme attached the following image(s):
IMG_5420.jpg (1,448kb) downloaded 185 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#19 Posted : 10/6/2018 5:02:19 PM

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so that one ended up being overrun with black mold (I parafilm plates like that, and discard them).
however, I saved the other honeydew transfer, the slow-growing sample on the left. Did a couple transfers from the academic strain on the right.
benzyme attached the following image(s):
IMG_5459.jpg (2,072kb) downloaded 153 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#20 Posted : 10/6/2018 5:16:37 PM

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here they are under 365nm light. There is a bacterial colony next to the wild specimen, in stasis. The plates are 0.05% chloramphenicol-PDA.
benzyme attached the following image(s):
IMG_5460.jpg (2,441kb) downloaded 148 time(s).
IMG_5461.jpg (3,089kb) downloaded 148 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
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