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The Cactus Analysis Thread Options
 
permatrip
#121 Posted : 12/10/2017 6:03:15 PM

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I have TLC plates from bunk Police and Marquis reagent. I need to get another reagent though since I want test cactus and try growing and breeding Illinois bundleflower for increased alkaloids. Probably best to do 2 reagent test even better 3 reagent tests as outlined in one of the booklets that came with the kit.

I got enough cactus powder I made by removing spines, cutting off strips removing the core. I intend on using my soxhlet and extract with either ethanol or methanol , I got both. Yea I bought 10 pounds I now know I can get much better cactus for the money.

I have chromatography paper from a lab supply company. I was hoping to be able to use it since it cost much less. I might find I can't use it though. I can afford to buy 10-20 tlc plates a month but I know others might not be able to, and that's why I asked about using paper. I will experiment with the paper when I do the tlc plates and see if any thing useful comes from trying paper, spraying with the ninhydrin solution.

I feel pretty confident that I will obtain better stock after reading many threads and the very useful info I found in this thread. I think of screening cactus and growing them as an investment in the future that will benefit more than just me. I feel like I'm involved in something much larger than myself. I got about 20 trichs I started from seed and will start another 20 soon. I know that 10 years from now I will be glad I started this project. Thanks to all of you for the ground work you have done that makes this possible for people like me. I am still learning so any advise is welcomed.

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An1cca
#122 Posted : 12/10/2017 9:24:03 PM

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permatrip wrote:
I took a sample to work with soon, I used a 5mm tube to get the sample, then froze and I think instead of using distilled water I will use the solvent provided with the kit. Methanol/ammonia solution. It seems to make sense to me.


Preliminary data suggest that taking the sample at the bottom 1/5 of the live stem provides the most accurate quantification of M-content.

Be sure that your samples are identical as to surface area of green flesh. Variations in thickness seem less important than variations in surface area.

Using the ammoniacal methanol will convert M to its free-base; lowering its solubility in the water that is contained in the flesh and that gets mixed up in your sample solution. Also, both M-freebase and its salts are soluble in water, why using methanol at this wet stage? It also has a lower boiling point making it boil away in a pressure cooker (while when using water this hardly plays a role).

permatrip wrote:
Instead of pressure cooking I intend on mashing the sample, sealing the eppindorf vial and in a glass container keep warm in a water bath for a lenght of time perhaps 8hr. Then extract using a filter draw into a syringe to load into a cap tube for TLC/test.


If you have a pressure cooker, I would say use it, be it for the sake of complete extraction and elimination of sliminess.

permatrip wrote:
I suppose I should do a second sample by freezing then pressure cooking the sample.

Thumbs up

permatrip wrote:
Has anyone any experience in testing using paper chromatography as paper is less expensive and was widely used and still is today as far as I read.

If you want to semi-quantify your extracts afterwards with ninhydrin, all lab-procedures use TLC. It has a better resolution and thus leaves a more localized and reproducible spot to analyse.

I'm so glad you're willing to peer-review the document.Love I'm sure it has value and together we can only further improve it. Thanks Permatrip and good luck. Be sure to post any results or questions.
 
permatrip
#123 Posted : 12/11/2017 5:36:13 AM

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Thanks An1cca, I will follow your instructions. I hadn't really thought it out, all you said makes sense. I thought extracting straight into the solvent system might help avoid the slimyness & avoid using the pressure cooker. I got a pc I'll put it to good use.

I just realized I am like the newbs I see all the time trying to grow their first mushrooms,not following tried and true methods that work. This was really useful to remind me not to try to innovate until I can create on my own. Don't know why I thought I could skip water extraction or pcing.

I find the separation kits to be inexpensive an a must have for serious cactus cultivators.

I realize I need to make a crystalline sample and get it tested to use as a reference in order to do anything worth posting about. Until then I'll research, read and live vicariously through you guys.
put your hands in my hand and together we will take on all the world
 
endlessness
#124 Posted : 10/16/2018 2:39:02 PM

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Just totally revamped the first post in this thread with a lot of updates, hopefully it will be more useful and organized now!

I'm also hoping more people will post their extraction results here (or here) so we can have better idea on cact yields and best cuttings.
 
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