  analytical chemist
 
     
 
 Posts: 7463 Joined: 21-May-2008 Last visit: 09-Aug-2025 Location: the lab 
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            new microscope, new photos. ~18X, sclerotia. notice the aspergillus mold spores. typically, sclerotia is soaked in isopropanol, then bleach, and cut with a scalpel then plated. benzyme attached the following image(s):   IMG_6611.jpg  (1,521kb) downloaded 155 time(s).  IMG_6612.jpg  (1,546kb) downloaded 149 time(s)."Nothing is true, everything is permitted." ~ hassan i sabbah "Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck 
   
         
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              analytical chemist
 
     
 
 Posts: 7463 Joined: 21-May-2008 Last visit: 09-Aug-2025 Location: the lab 
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            might someone be able to access this  https://www.sciencedirec...pii/B9780123979407000100my current work is with DNA extraction and amplification, and I work alongside geneticists. I'll get some insight on methods to initiate DMATs, in a strain that's reverted back to wild-type, and post my findings.  and before anyone asks/posts, yeah I already have these. https://www.sciencedirec...bs/pii/S0031942209002155https://www.sciencedirec...bs/pii/S0734975012000079"Nothing is true, everything is permitted." ~ hassan i sabbah "Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck 
   
         
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              analytical chemist
 
     
 
 Posts: 7463 Joined: 21-May-2008 Last visit: 09-Aug-2025 Location: the lab 
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            nm. got it. "Nothing is true, everything is permitted." ~ hassan i sabbah "Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck 
   
         
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              analytical chemist
 
     
 
 Posts: 7463 Joined: 21-May-2008 Last visit: 09-Aug-2025 Location: the lab 
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              "Nothing is true, everything is permitted." ~ hassan i sabbah "Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck 
   
         
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              In the gap between thoughts nonconceptual wisdom shines continuously.
 
 
 Posts: 207 Joined: 16-Sep-2017 Last visit: 11-Mar-2024 Location: ⚗ alembic ⚗ 
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              DMT-Nexus member
 
 
 Posts: 1285 Joined: 23-Jun-2018 Last visit: 22-Feb-2022 
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            Benz.  I'm sorry to necro your thread.  Do I understand correctly... Claviceps is a fungus, so from that sclerotia you were growing claviceps mycelium on those agar plates?? Like cloning a mushroom?  olympus mon wrote:You need to hit it with intention to get where you want to be!  "Good and evil lay side by side as electric love penetrates the sky..." -Hendrix"We have arrived at truth, and now we find truth is a mystery- a play of joy, creation, and energy.  This is  source. This is the mystic touchstone that heals and renews.  This is the beginning again.  This is entheogenic."  -Nicholas Sand  
         
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              analytical chemist
 
     
 
 Posts: 7463 Joined: 21-May-2008 Last visit: 09-Aug-2025 Location: the lab 
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            I attempted it, but it stalled on chloramphenicol-PDA plates. What did work well was plating the honeydew from those wild specimens. "Nothing is true, everything is permitted." ~ hassan i sabbah "Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck 
   
         
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