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benzyme
#1 Posted : 9/12/2018 2:20:32 AM

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From what I've seen, there aren't many close up photos (besides microscopic) on the webs. I just collected these specimens, not even an hour ago. The close-up middle of the top photo is newly formed sclerotia.
Still early in the season, they typically form hardened brown/black brain-like sclerotia when the weather gets cold in late fall/early winter.
benzyme attached the following image(s):
IMG_5305.jpg (2,175kb) downloaded 291 time(s).
IMG_5304.jpg (2,661kb) downloaded 289 time(s).
IMG_5309.jpg (2,945kb) downloaded 284 time(s).
IMG_5307.jpg (2,128kb) downloaded 284 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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Ever have a personal encounter with an entity after taking DMT?
 
dragonrider
#2 Posted : 9/12/2018 11:05:37 AM

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Are you gonna make yourself some kykeon benz?

But seriously, how is it that all naturally occurring lysergamides come from fungi that grow on the seeds of plants? Is claviceps related to the fungi on morning glory and hawaian baby woodrose, or is it just something in the biochemistry of fungi in general that makes fungi produce lysergamides, but not plants or algea?
 
benzyme
#3 Posted : 9/12/2018 12:54:07 PM

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first, I’ll plate honeydew; and get some pics of the organism under the [compound] microscope.
Then sterilize the sclerotia in 15% saline, and cut slerotia, then plate those. after a couple weeks, I’d do a couple transfers, and do characterization under UV, and some preliminary Van urk’s reagent tests. if they check out, onto fermentation of secondary metabolites.

I don’t know the relation to endophytic fungi found in HBWR and MG; they may be distant cousins. their mechanism is the same: hijack tryptophan, and use it in the DMATS pathway. Humidity and temp tend to influence the levels of alk production of the fungus on live plants.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
downwardsfromzero
#4 Posted : 9/12/2018 3:29:00 PM

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dragonrider wrote:
how is it that all naturally occurring lysergamides come from fungi that grow on the seeds of plants?

Footnote here: Some races of Lolium arundinaceum syn. Festuca arundinacea (tall fescue) contain ergoline alkaloids, principally ergovaline, in the leaves. I haven't found anything about the alkaloid content of its seeds yet. Epichloë coenophiala is the endophyte responsible for alkaloid production in this instance. (Published yields would suggest that tall fescue is not a viable source species for ergoline alkaloids.)

benzyme wrote:
Humidity and temp tend to influence the levels of alk production of the fungus on live plants.

Me being lazy and all, do you have a good reference for some specific data on this?

Great project, looking forward to further reports Thumbs up
Ora, lege, lege, lege, relege et labora

“To be GOVERNED is to be watched, inspected, spied upon, directed, law-driven, numbered, regulated, enrolled, indoctrinated, preached at, controlled, checked, estimated, valued, censured, commanded, by creatures who have neither the right nor the wisdom nor the virtue to do so. To be GOVERNED is to be at every operation, at every transaction noted, registered, counted, taxed, stamped, measured, numbered, assessed, licensed, authorized, admonished, prevented, forbidden, reformed, corrected, punished. It is, under pretext of public utility, and in the name of the general interest, to be place under contribution, drilled, fleeced, exploited, monopolized, extorted from, squeezed, hoaxed, robbed; then, at the slightest resistance, the first word of complaint, to be repressed, fined, vilified, harassed, hunted down, abused, clubbed, disarmed, bound, choked, imprisoned, judged, condemned, shot, deported, sacrificed, sold, betrayed; and to crown all, mocked, ridiculed, derided, outraged, dishonored. That is government; that is its justice; that is its morality."
― Pierre-Joseph Proudhon
 
benzyme
#5 Posted : 9/12/2018 4:32:16 PM

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http://b-ok.xyz/book/510008/a93655


"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
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benzyme
#6 Posted : 9/13/2018 3:42:23 AM

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ahh, I missed the part where they are referring to rye.

anywho, if you find any interesting info specific to paspali in that book, please post. I plan to generate a crap-ton of data.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
downwardsfromzero
#7 Posted : 9/13/2018 2:57:47 PM

Eggnogstic - warning! Self-irony levels: high!

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Thanks for the link.

I'll continue with my general-purpose data scouring and will be sure to post any relevant comments brought up.
Ora, lege, lege, lege, relege et labora

“To be GOVERNED is to be watched, inspected, spied upon, directed, law-driven, numbered, regulated, enrolled, indoctrinated, preached at, controlled, checked, estimated, valued, censured, commanded, by creatures who have neither the right nor the wisdom nor the virtue to do so. To be GOVERNED is to be at every operation, at every transaction noted, registered, counted, taxed, stamped, measured, numbered, assessed, licensed, authorized, admonished, prevented, forbidden, reformed, corrected, punished. It is, under pretext of public utility, and in the name of the general interest, to be place under contribution, drilled, fleeced, exploited, monopolized, extorted from, squeezed, hoaxed, robbed; then, at the slightest resistance, the first word of complaint, to be repressed, fined, vilified, harassed, hunted down, abused, clubbed, disarmed, bound, choked, imprisoned, judged, condemned, shot, deported, sacrificed, sold, betrayed; and to crown all, mocked, ridiculed, derided, outraged, dishonored. That is government; that is its justice; that is its morality."
― Pierre-Joseph Proudhon
 
dragonrider
#8 Posted : 9/14/2018 8:09:45 PM

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It is a while ago now, but on a couple of occasions i have taken 1P-LSD in conjunction with some morning glory seeds.

The very odd thing was...when the seeds where old, the effects of the 1P-LSD where dulled, but with fresh seeds the effects where being amplified...significantly. It went as far as having OBE's, wich i never had with LSD by itself.

It may seem as if the degradation of LSH into LSA, is responsible for this. But i wonder if there may be other substances in these seeds, and probably the claviceps species as well, that could explain the very extreme synergistic effects i experienced.

Are there lysergamides, that modulate receptors that LSD is active on?
 
benzyme
#9 Posted : 9/14/2018 8:32:10 PM

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in short, yes..with varying degrees of agonism/antagonism. Ergoline pharmacology is rather complex, given the variety of structures and their different binding affinities on the various 5HT-subtypes
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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benzyme
#10 Posted : 9/14/2018 9:51:26 PM

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Cool
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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benzyme
#11 Posted : 9/14/2018 10:23:15 PM

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This is the academic (ATCC) strain, from the original line I worked with in 2011. I kept it in the freezer since then.
Just plated this last week, on plain-jane PDA. I prepped more PDA plates today, with chloramphenicol, for transfers.

benzyme attached the following image(s):
IMG_5318.jpg (2,434kb) downloaded 195 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
benzyme
#12 Posted : 9/16/2018 1:33:13 AM

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http://b-ok.xyz/book/2264805/fffb0b
benzyme attached the following image(s):
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7DC50E81-47F7-4A63-B59D-335E5CBB8F46.png (913kb) downloaded 182 time(s).
3997A1B2-1023-4E12-9B5B-CF745181A216.png (1,239kb) downloaded 182 time(s).
7037329D-5987-436B-B1D2-598E429A64F4.png (1,724kb) downloaded 180 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
dragonrider
#13 Posted : 9/16/2018 12:04:04 PM

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benzyme wrote:
in short, yes..with varying degrees of agonism/antagonism. Ergoline pharmacology is rather complex, given the variety of structures and their different binding affinities on the various 5HT-subtypes

That is very interesting. I was planning to do some more experiments with combining lysergamides, but the times it DID work, the effects where so extreme and unpredictable that it frightened me a little.

But maybe i should not let that put me off. If i can get my hands on some fresh seeds again, wich shouldn't be too difficult.....

If we would know more about how this works exactly, wich chemicals in wich quantities, etc, the consequences could be significant. It's like adding a whole new dimension to the LSD experience.
 
benzyme
#14 Posted : 9/16/2018 2:59:20 PM

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check the link above those pages I uploaded, your question was what prompted me to link that book . It is pharmacologically comprehensive.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
dragonrider
#15 Posted : 9/16/2018 4:56:38 PM

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OK thanks.
 
benzyme
#16 Posted : 9/22/2018 3:34:39 AM

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here's a better pic of a new sclerotial cell. collected it from a trail/pond in my neighborhood. there's another underneath, in the middle.
benzyme attached the following image(s):
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"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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benzyme
#17 Posted : 9/23/2018 8:17:40 PM

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major score today. lots of sclerotia.
plated some honeydew on chloramphenicol-PDA, after my last attempts ended up with bacterial colonies.

benzyme attached the following image(s):
IMG_5412.jpg (1,773kb) downloaded 116 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
benzyme
#18 Posted : 9/26/2018 11:22:10 PM

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after three days, this is what I got. Purple sectors are high-producers, from what I've read. the plate is clean, with chloramphenicol, it better be.
the dark spots are just carbon deposits, from the flame-sterilized tweezers.
benzyme attached the following image(s):
IMG_5420.jpg (1,448kb) downloaded 89 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
benzyme
#19 Posted : 10/6/2018 5:02:19 PM

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so that one ended up being overrun with black mold (I parafilm plates like that, and discard them).
however, I saved the other honeydew transfer, the slow-growing sample on the left. Did a couple transfers from the academic strain on the right.
benzyme attached the following image(s):
IMG_5459.jpg (2,072kb) downloaded 56 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
BTC 3CNrz6Tj17U35GLkRB1zsNtVoedorw8oja
ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
benzyme
#20 Posted : 10/6/2018 5:16:37 PM

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here they are under 365nm light. There is a bacterial colony next to the wild specimen, in stasis. The plates are 0.05% chloramphenicol-PDA.
benzyme attached the following image(s):
IMG_5460.jpg (2,441kb) downloaded 51 time(s).
IMG_5461.jpg (3,089kb) downloaded 52 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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ETH 0xd433198cb786145767c67d874b36cd7d42baf57a
 
 
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