It's in literature several times that they have a significantly different Rf, benz.

Check this file I uploaded here with the Rfs of different alkaloids according to literature: https://www.dmt-nexus.me...aspx?g=posts&t=27459

Of course for a definite identification one would need mass-spec, but if making n-oxide works as according to literature with hydrogen peroxide, if the Rf's match, and if when testing some plant material you see spot at same height and changing to the same color with some reagent, its very very good possibility.. And if its not there at all, well its also very likely dmt n-oxide is not present at all.. Dont you agree?

fourthripley, did you every bioassay the arundinacea extracts? My own experience with extracting and testing(on myself) wild arundinacea was similar to what you described of your experience with brachy's..only I never went quite as far as you did. I tested lighter doses a few times and there was definatily tryptamine effects pesent..Only once did I smoke a bit too much and this was very much like what I have read of 5meoDMT..it was frightening but facinating. I have alot of experience with DMT but none with pure 5meoDMT though so I cant be 100% sure what it was that caused that effect.

Interesting that you find an increase in alkaloids in grass harvested in the fall..that has been my observation as well with wild grass.

I must say that these reports of grass extractions that include bioassays are worth so much..too often a thread comes up with some extraction pics etc..someone extracts a yellow goo or something and then the thread just dies..no bioassay..which just sort leaves us back where we started. We all know grass can yield a yellow goo..but analysis and bioassay are what we need.

Yep thanks for posting this. Just found a bag of Phalaris brachystachys recently I will start to grow them soon...

I am interested in more natural extraction tek, but it seems the variability means that some kind of extraction is needed to determine potency.

Ultimately I would like to use it as a dmt source for ayahuasca, that I can grow in the UK.

To further get a feel for the alkaloid profile/amounts and determine the usefulness of this plant I decided to conduct some oral + maoi experiments.
150mg of Manske extracted harmaloids was disolved in warm water and drunk and the juice of 30g wet foliage consumed 20 minutes later. I felt I may have detected
a faint effect not totally attributable to the harmaloids, but too weak that it couldn't be put down to placebo.
For the next experiment, thinking I may have underdosed on the maoi component for my body mass, I upped the dose to 250mg.
At 12.00 today I consumed the above followed 20 minutes later with the juiceof 60g foliage.If in doubt double the dose...
12.30: first alert
12.45: a definite effect, resigned to things dropping off here but hoping they don't.
12.55: this is not a dud. A moderately strong dmt effect. Only minor visual distortions and no patterns but an unmistakeable positive. Everything has 'the look'. Difficult to describe, a very organic and 'clean' feeling. Euphoria. I would maybe compare it to the 5 minutes after vaping 50mg of fb, only without the 'weight', and much extended over time. A plateau over a period of an hour with waves of increasing intensity gently lapping throughout.
14.00: rapidly drops away and I feel able to engage in light conversation. Feeling great, with colours still pleasantly enhanced with occasional echoes of the peak. The past hour and a half subjectively much longer than it was.
14.30: baseline. Negotiated a busy supermarket without incident.

Can we be sure that there is not any DMT in the plant itself already in the form of oxides? The np solvent used in these teks was naptha..which wont pull much if any oxides. The yields do seem lower than what should be expected of brachys. I wonder also if maybe some of the DMT could be bound up in something else similar to with mimosa..where xylene etc would pull a larger yield..

DCM third pulls performed on the cold basic solution yield very modest amounts, what I would expect from a third pull. However, DCM does pull something else from the basic solution that naphtha does not; dried DCM extractions have a strong smell I would describe as simiar to rotting leaves or Ivy in flower. I wonder if this might be gramine or some other less desirable product. I haven't been able to find a desciption anywhere of what Gramine might smell like.