CHATPRIVACYDONATELOGINREGISTER
DMT-Nexus
FAQWIKIHEALTH & SAFETYARTATTITUDEACTIVE TOPICS
Rf for different alkaloids (TLC/Paper/Column chromatography) Options
 
endlessness
#1 Posted : 11/28/2011 6:09:11 PM

DMT-Nexus member

Moderator

Posts: 13502
Joined: 19-Feb-2008
Last visit: 21-Nov-2019
Location: Jungle
Im uploading an excel table i've been working on (thanks for the help global & doingkermit), with Rfs for different substances on a variety of different solvent systems (150+).

All the table includes references with hyperlink to the full publication for easy source-checking, was quite some work but I think its worth it Smile

So far (28/11) the substances added are : NMT, DMT, DMT N-Oxide, psilocin, psilocybin, bufotenine, bufotenine n-oxide, bufoviridine, dehydrobufotenine, 5-MeO-NMT, 5-MeO-DMT, 5-MeO-DMT n-oxide, gramine, tryptamine, harmine, harmaline, THH, DPT, DET.

Ideally this table will grow with more publications I find and help of others around the Nexus.

Please feel free to give any suggestions, comments, or whatever. I hope the file is working well.
 

STS is a community for people interested in growing, preserving and researching botanical species, particularly those with remarkable therapeutic and/or psychoactive properties.
 
endlessness
#2 Posted : 11/28/2011 9:27:17 PM

DMT-Nexus member

Moderator

Posts: 13502
Joined: 19-Feb-2008
Last visit: 21-Nov-2019
Location: Jungle
Ok all the publications I could find are uploaded, file updated. I will reupload as more information is gathered, and the filename will have the date of the update, so people can know if they got the latest file or not.

If anybody has any pointers on publications that have Rf of different substances, whether they are tryptamines or any other drugs, feel free to tell me the reference so i can add to the file (or add to the file yourself)
 
InMotion
#3 Posted : 11/28/2011 9:30:15 PM
DMT-Nexus member

Senior Member | Skills: 3D programming, Mathematician (if you need help with algebra to differential equations I'm available), SKilled Engineer

Posts: 473
Joined: 07-Aug-2011
Last visit: 10-Jan-2014
Thank you end Smile very valuable information.
 
Dozuki
#4 Posted : 11/28/2011 10:01:48 PM

Faustian Phytochem Investigator

Chemical expertSenior Member

Posts: 162
Joined: 31-Oct-2011
Last visit: 30-Mar-2017
Location: Oaxaca
Funny, I did the same thing this weekend, only on paper... Not sure I have a copy of Exel but will cross reference once I can check out the file.

-D.
 
Mindlusion
#5 Posted : 11/28/2011 10:07:26 PM

Chairman of the Celestial Divison

Extreme Chemical expertChemical expertSenior Member

Posts: 1271
Joined: 21-Jul-2010
Last visit: 21-Nov-2019
Location: the ancient cluster
sweet. Smile
Expect nothing, Receive everything.
"Experiment and extrapolation is the only means the organic chemists (humans) currrently have - in contrast to "God" (and possibly R. B. Woodward). "
He alone sees truly who sees the Absolute the same in every creature...seeing the same Absolute everywhere, he does not harm himself or others. - The Bhagavad Gita
"The most beautiful thing we can experience, is the mysterious. The source of all true art and science."
 
Tannenberg
#6 Posted : 6/16/2012 12:30:09 AM

DMT-Nexus member


Posts: 49
Joined: 27-Feb-2012
Last visit: 28-Dec-2013
Dozuki wrote:
Funny, I did the same thing this weekend, only on paper... Not sure I have a copy of Exel but will cross reference once I can check out the file.

-D.


I generated a pdf from the exel file for you (i hope this is ok for you endlessness)
 
Poekus
#7 Posted : 3/30/2013 9:56:32 PM
DMT-Nexus member


Posts: 353
Joined: 03-Nov-2010
Last visit: 14-Mar-2018
Location: Nl
Here is something I found about TLC with Rf's for identifying mescaline which could be useful for the excel.
Maybe it's already posted somewhere here on the forum but I couldn't find it with the search.

It's coming from linked source which also has several other methods of mescaline identification described. According to the pdf it's referenced from
J. "Clarke's Isolation and Identification of Drugs"': 2nd edifion, The Pharmaceutical Press (London) 1986.

The Analysis of Peyote Cactus, Mescal Buttons and Mescaline


E. Thin-layer chromatography
Plates:
Activated silica gel G on glass backed plates; the coating (0.25 mm thickness) contains a fluorescing additive which fluoresces at 254 nm.

Developing solvents:
System A: Chloroform 82 Methanol 17
Concentrated ammonia solution 1 System B: Methanol 100
Concentrated ammonia solution 1.5 Preparation of solutions to be applied to the TLC plate:

a) Peyote cactus, Mescal Buttons:
About 10 mg of a representative sample of the suspected dried and pulverized
plant material is extracted using the method outlined in Chapter B. If using ninhydrin reagent for visualization the extract should be concentrated to approxymately 200 ul under a stream of nitrogen. and 10 ul of the filtered extract are spotted onto the TLC plate.

b) Mescaline powder:
Prepare a solution at a concentration of approximately 1 mg/ml methanol as
outlined in Chapter B. and spot 1 ul of this solution onto the TLC plate.

c) Mescaline tablets and capsules:
Extract the material using method outlined in Chapter B. and spot 10 ul of this
extract onto TLC plate. d) Standard solution:
Prepare a solution of 1 mg/ml mescaline sulfate or hydrochloride methanol and spot 1 ul of this standard solution onto the TLC plate.

Visualization
The plates must be dried prior to visualization. This can be done by air-drying at room temperature or by short use of a hot air blower.

Visualization methods:
1. UV light at 254 nm.
2. Fluorescamine (Fluram) reagent 3. Ninhydrin reagent.
Spray reagents:
(1) Fluorescamine reagent: Prepare a solution of 10 mg fluorescamine in 50ml
dried acetone.
(2) Ninhydrin reagent: Prepare a 10% solution of ninhydrin in ethanol.

Method:
Observe first the plate under UV light at 254 nm. Mescaline will absorb the light
and appear as a dark spot on the fluorescent background. Then spray with reagent(1) or (2). When using reagent (1), the plate has to be dried after spraying by shortly using a hot air blower. Then observe the plate under UV light at 365 nm.
Mescaline gives a bright yellow fluorescent spot. The detection limit is about 10 ng mescaline. The fluorescence can be intensified by exposing the plate to ammonia vapour. When using reagent (2) the plate has to be heated after spraying in an oven at 120°C for at least 15 minutes. A violet spot is given by mescaline. The detection limit is about 1 ug mescaline.

Results

With developing systems A and B, mescaline gives spots at Rf x 100 value
approximating 36 and 24, respectively.
 
fastfred
#8 Posted : 6/3/2013 9:39:06 PM
DMT-Nexus member


Posts: 57
Joined: 03-Jun-2013
Last visit: 24-Sep-2013
Apparently I don't have access to the file... [Edit: Got access, thanks!]

Here is a reference I have on a few of the interesting molecules, with a good number of solvent systems...

-FF
fastfred attached the following image(s):
TLC_Chart.gif (63kb) downloaded 486 time(s).
 
Tana
#9 Posted : 9/14/2013 10:13:59 AM
DMT-Nexus member

New member

Posts: 4
Joined: 11-Sep-2012
Last visit: 12-Aug-2018
I don't write english as good as I read, then I will just post this PDF that I am sure that everyone saw once in a life, but helped me a lot:

 
pitubo
#10 Posted : 2/26/2015 12:02:51 AM

dysfunctional word machine

Senior Member

Posts: 1831
Joined: 15-Mar-2014
Last visit: 11-Jun-2018
Location: at the center of my universe
Bleach is cheap and commonly available. The authors of the below article describe its use as a sensitive TLC stain.

Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection
Noriyuki Kato, Takashi Kojima, Shinji Yoshiyagawa, Hikoto Ohta, Akira Toriba, Hideo Nishimura, Kazuichi Hayakawa
Journal of Chromatography A Volume 1145, Issues 1–2, 23 March 2007, Pages 229–233

Abstract
A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365 nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01 μg to 0.06 μg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers’ urine samples.

http://dx.doi.org/10.1016/j.chroma.2007.01.061
 
 
Users browsing this forum
Guest

DMT-Nexus theme created by The Traveler
This page was generated in 0.149 seconds.