Rf for different alkaloids (TLC/Paper/Column chromatography) Options
#1 Posted : 11/28/2011 6:09:11 PM

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Im uploading an excel table i've been working on (thanks for the help global & doingkermit), with Rfs for different substances on a variety of different solvent systems (150+).

All the table includes references with hyperlink to the full publication for easy source-checking, was quite some work but I think its worth it Smile

So far (28/11) the substances added are : NMT, DMT, DMT N-Oxide, psilocin, psilocybin, bufotenine, bufotenine n-oxide, bufoviridine, dehydrobufotenine, 5-MeO-NMT, 5-MeO-DMT, 5-MeO-DMT n-oxide, gramine, tryptamine, harmine, harmaline, THH, DPT, DET.

Ideally this table will grow with more publications I find and help of others around the Nexus.

Please feel free to give any suggestions, comments, or whatever. I hope the file is working well.

STS is a community for people interested in growing, preserving and researching botanical species, particularly those with remarkable therapeutic and/or psychoactive properties.
#2 Posted : 11/28/2011 9:27:17 PM

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Ok all the publications I could find are uploaded, file updated. I will reupload as more information is gathered, and the filename will have the date of the update, so people can know if they got the latest file or not.

If anybody has any pointers on publications that have Rf of different substances, whether they are tryptamines or any other drugs, feel free to tell me the reference so i can add to the file (or add to the file yourself)
#3 Posted : 11/28/2011 9:30:15 PM
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Thank you end Smile very valuable information.
#4 Posted : 11/28/2011 10:01:48 PM

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Funny, I did the same thing this weekend, only on paper... Not sure I have a copy of Exel but will cross reference once I can check out the file.

#5 Posted : 11/28/2011 10:07:26 PM

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sweet. Smile
Expect nothing, Receive everything.
"Experiment and extrapolation is the only means the organic chemists (humans) currrently have - in contrast to "God" (and possibly R. B. Woodward). "
He alone sees truly who sees the Absolute the same in every creature...seeing the same Absolute everywhere, he does not harm himself or others. - The Bhagavad Gita
"The most beautiful thing we can experience, is the mysterious. The source of all true art and science."
#6 Posted : 6/16/2012 12:30:09 AM

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Dozuki wrote:
Funny, I did the same thing this weekend, only on paper... Not sure I have a copy of Exel but will cross reference once I can check out the file.


I generated a pdf from the exel file for you (i hope this is ok for you endlessness)
#7 Posted : 3/30/2013 9:56:32 PM
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Here is something I found about TLC with Rf's for identifying mescaline which could be useful for the excel.
Maybe it's already posted somewhere here on the forum but I couldn't find it with the search.

It's coming from linked source which also has several other methods of mescaline identification described. According to the pdf it's referenced from
J. "Clarke's Isolation and Identification of Drugs"': 2nd edifion, The Pharmaceutical Press (London) 1986.

The Analysis of Peyote Cactus, Mescal Buttons and Mescaline

E. Thin-layer chromatography
Activated silica gel G on glass backed plates; the coating (0.25 mm thickness) contains a fluorescing additive which fluoresces at 254 nm.

Developing solvents:
System A: Chloroform 82 Methanol 17
Concentrated ammonia solution 1 System B: Methanol 100
Concentrated ammonia solution 1.5 Preparation of solutions to be applied to the TLC plate:

a) Peyote cactus, Mescal Buttons:
About 10 mg of a representative sample of the suspected dried and pulverized
plant material is extracted using the method outlined in Chapter B. If using ninhydrin reagent for visualization the extract should be concentrated to approxymately 200 ul under a stream of nitrogen. and 10 ul of the filtered extract are spotted onto the TLC plate.

b) Mescaline powder:
Prepare a solution at a concentration of approximately 1 mg/ml methanol as
outlined in Chapter B. and spot 1 ul of this solution onto the TLC plate.

c) Mescaline tablets and capsules:
Extract the material using method outlined in Chapter B. and spot 10 ul of this
extract onto TLC plate. d) Standard solution:
Prepare a solution of 1 mg/ml mescaline sulfate or hydrochloride methanol and spot 1 ul of this standard solution onto the TLC plate.

The plates must be dried prior to visualization. This can be done by air-drying at room temperature or by short use of a hot air blower.

Visualization methods:
1. UV light at 254 nm.
2. Fluorescamine (Fluram) reagent 3. Ninhydrin reagent.
Spray reagents:
(1) Fluorescamine reagent: Prepare a solution of 10 mg fluorescamine in 50ml
dried acetone.
(2) Ninhydrin reagent: Prepare a 10% solution of ninhydrin in ethanol.

Observe first the plate under UV light at 254 nm. Mescaline will absorb the light
and appear as a dark spot on the fluorescent background. Then spray with reagent(1) or (2). When using reagent (1), the plate has to be dried after spraying by shortly using a hot air blower. Then observe the plate under UV light at 365 nm.
Mescaline gives a bright yellow fluorescent spot. The detection limit is about 10 ng mescaline. The fluorescence can be intensified by exposing the plate to ammonia vapour. When using reagent (2) the plate has to be heated after spraying in an oven at 120°C for at least 15 minutes. A violet spot is given by mescaline. The detection limit is about 1 ug mescaline.


With developing systems A and B, mescaline gives spots at Rf x 100 value
approximating 36 and 24, respectively.
#8 Posted : 6/3/2013 9:39:06 PM
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Apparently I don't have access to the file... [Edit: Got access, thanks!]

Here is a reference I have on a few of the interesting molecules, with a good number of solvent systems...

fastfred attached the following image(s):
TLC_Chart.gif (63kb) downloaded 533 time(s).
#9 Posted : 9/14/2013 10:13:59 AM
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I don't write english as good as I read, then I will just post this PDF that I am sure that everyone saw once in a life, but helped me a lot:

#10 Posted : 2/26/2015 12:02:51 AM

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Bleach is cheap and commonly available. The authors of the below article describe its use as a sensitive TLC stain.

Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection
Noriyuki Kato, Takashi Kojima, Shinji Yoshiyagawa, Hikoto Ohta, Akira Toriba, Hideo Nishimura, Kazuichi Hayakawa
Journal of Chromatography A Volume 1145, Issues 1–2, 23 March 2007, Pages 229–233

A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365 nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01 μg to 0.06 μg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers’ urine samples.
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