We've Moved! Visit our NEW FORUM to join the latest discussions. This is an archive of our previous conversations...

You can find the login page for the old forum here.
CHATPRIVACYDONATELOGINREGISTER
DMT-Nexus
FAQWIKIHEALTH & SAFETYARTATTITUDEACTIVE TOPICS
12NEXT
DIY Thin Layer Chromatography Options
 
benzyme
#1 Posted : 1/2/2011 11:49:46 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab
whether one is extracting alkaloids from seeds or plant material, it helps to have a means of analysis, and a reference to compare it against. attached are a tutorial to make your own TLC plates, and a chapter on alkaloids from 'Plant Drug Analysis-A Thin Layer Chromatography Atlas'
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 

STS is a community for people interested in growing, preserving and researching botanical species, particularly those with remarkable therapeutic and/or psychoactive properties.
 
actualfactual
#2 Posted : 1/3/2011 12:02:26 AM

DMT-Nexus member


Posts: 681
Joined: 11-Sep-2010
Last visit: 24-Dec-2011
good info. thanks.
 
Infundibulum
#3 Posted : 1/3/2011 12:17:09 AM

Kalt und Heiß, Schwarz und Rot, Kürper und Geist, Liebe und Chaos

ModeratorChemical expert

Posts: 4661
Joined: 02-Jun-2008
Last visit: 30-Apr-2022
Amazing stuff!

This is great for someone who wishes to test their local flora for dmt.

Need to calculate between salts and freebases? Click here!
Need to calculate freebase or salt percentage at a given pH? Click here!

 
Adivino
#4 Posted : 1/3/2011 12:51:16 AM

DMT-Nexus member


Posts: 154
Joined: 03-May-2010
Last visit: 18-Dec-2013
Location: Under the sea
In case one wanted to get the book, where was the second pdf taken from?
The text above was typed by frenzy monkeys randomly hitting their keyboards.
 
benzyme
#5 Posted : 1/3/2011 12:54:46 AM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab
http://ebooklink.net/g/d...0Chromatography%20Atlas/
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#6 Posted : 1/3/2011 1:16:01 AM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab
bam.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
Shaolin
#7 Posted : 1/3/2011 10:48:40 AM

Stiletto Stoner

Moderator

Posts: 1132
Joined: 18-Nov-2008
Last visit: 15-Mar-2015
Location: Blazin'
Erowid - for 5-MEO but a nice overview.

Also Wizard-X to the rescue. X-file 7.

Got GVG ? Mhm. Got DMT ?

Pandora wrote:
Nexus enjoys cutting edge and ongoing superior programming skills of the owner of this site (The Traveler), including recent switching to the .me domain name.


I'm still, I'm still Jenny from the block

Simon Jester wrote:
"WTF n00b, buy the $100 vapor pipe or GTFO"


Ignorance of the law does not protect you from prosecution
 
The Traveler
#8 Posted : 1/4/2011 10:25:48 PM

"No, seriously"

Administrator | Skills: DMT, LSD, Programming

Posts: 7324
Joined: 18-Jan-2007
Last visit: 27-Mar-2024
Location: Orion Spur
I see in this place that you can buy a roll of 1" x 100yd of Chromatography paper (you need about 8 inch of paper per test, so 450 tests on this roll) for only USD 25,75: click!

Benzyme also told me in the chat that you need:
* ethyl acetate and methanol (or ethanol/IPA instead of methanol) as the Eluent, the solvent being used to push the mixture
* UV light at about 300nm
* pipet or eye dropper to make the tiny droplet on the paper
* A jar that can be closed as the 'reagent chamber'
* Preferable also a reagent spray (it's not really needed but it can make the results more clear)

Also I like to point out that TLC is a qualitative and not quantitative measurement. So it gives you the relative abundance info, qualitatively. It's not to measure the percentages of the different contents.


Kind regards,

The Traveler
 
benzyme
#9 Posted : 1/5/2011 12:13:13 AM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab
yes

this is good stuff.
the implication is simple, folks: the future availability of root bark is questionable.

it seems like a good time to look at other plant sources. TLC/paper chrom are easy methods of
characterizing the different alkaloids. once one gets the hang of this method, column chromatography is the next step...sample preparation.
the diagram posted by Shaolin is a good one. it's vacuum column chromatography.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
Shaolin
#10 Posted : 1/5/2011 11:37:41 AM

Stiletto Stoner

Moderator

Posts: 1132
Joined: 18-Nov-2008
Last visit: 15-Mar-2015
Location: Blazin'
The Home Scientist 024 - Making thin-layer chromatography (TLC) plates

Got GVG ? Mhm. Got DMT ?

Pandora wrote:
Nexus enjoys cutting edge and ongoing superior programming skills of the owner of this site (The Traveler), including recent switching to the .me domain name.


I'm still, I'm still Jenny from the block

Simon Jester wrote:
"WTF n00b, buy the $100 vapor pipe or GTFO"


Ignorance of the law does not protect you from prosecution
 
burnt
#11 Posted : 1/5/2011 5:38:58 PM

DMT-Nexus member

Extreme Chemical expertChemical expertSenior Member

Posts: 3555
Joined: 13-Mar-2008
Last visit: 19-Aug-2020
Location: not here
While I'd certainly encourage do it yourself TLC just one note. Silica dust is toxic. It will get in your body and stay there. Its kind of like breathing asbestos. So just make sure to wear mask when making your own TLC plates or cutting up TLC plates. Also work in an area you can easily clean etc usual lab stuff.
 
Aegle
#12 Posted : 1/7/2011 3:44:12 PM

Cloud Whisperer

Senior Member | Skills: South African botanicals, Mushroom cultivator, Changa enthusiast, Permaculture, Counselling, Photography, Writing

Posts: 1953
Joined: 05-Jan-2009
Last visit: 22-Jan-2020
Location: Amongst the clouds
Benzyme

Nice this thread is awesome, shot a lot. Cool


Much Peace and Rainbows
The Nexus Art Gallery | The Nexian | DMT Nexus Research | The Open Hyperspace Traveler Handbook

For small creatures such as we the vastness is bearable only through love.

The fate of our times is characterised by rationalisation and intellectualisation and, above all, by the disenchantment of the world.

Following a Path of Compassion and Heart
 
ragabr
#13 Posted : 3/26/2011 4:13:31 PM

DMT-Nexus member


Posts: 2354
Joined: 24-Jan-2010
Last visit: 21-Jun-2012
Location: Massachusetts
SWIM is planning on starting up a bunch of Illinois Bundleflower seeds, and was hoping to use this to determine which plants to save seeds from. It looks like the only appropriate method given in 'Plant Drug Analysis-A Thin Layer Chromatography Atlas' is method C, but I don't think di-ethyl ether will be easily available to her.

Does this even seem like a reasonable approach, and might there be a more readily available substitute for the ether?
PK Dick is to LSD as HP Lovecraft is to Mushrooms
 
benzyme
#14 Posted : 3/26/2011 4:47:55 PM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab


it's roughly 60/40 ether/heptane
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
rOm
#15 Posted : 3/26/2011 7:07:19 PM

DMT-Nexus member

Senior Member

Posts: 2096
Joined: 20-Nov-2009
Last visit: 12-Nov-2023
Thanks a lot for the infos BEnz and al.
Smell like tea n,n spirit !

Toke the toke, and walk the walk !
 
Dr_Sister
#16 Posted : 4/19/2011 8:44:58 PM

Synaptic cleft explorer

Chemical expert | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, MarketingSenior Member | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, Marketing

Posts: 299
Joined: 10-Dec-2010
Last visit: 13-Feb-2014
Location: good question
Yes, very informative thread!

Question: To visualize dmt or yuremamine would a UV light be sufficient or would a stain of some sort be required? If a stain is required, would iodine vapour or vanillin/EtoH/H2SO4 or perhaps even dilute H2SO4 stains work for this?

Thanks
Sister
 
benzyme
#17 Posted : 4/20/2011 6:29:59 AM

analytical chemist

Moderator | Skills: Analytical equipment, Chemical master expertExtreme Chemical expert | Skills: Analytical equipment, Chemical master expertChemical expert | Skills: Analytical equipment, Chemical master expertSenior Member | Skills: Analytical equipment, Chemical master expert

Posts: 7463
Joined: 21-May-2008
Last visit: 03-Mar-2024
Location: the lab
Ehrlich's reagent (aka Van Urk's) is a qualitative test for the presence of indole alkaloids; the azide complex it forms results in a characteristic color for each respective alkaloid.

it is p-dimethylaminobenzaldehyde in either 2% H2SO4 or HCl.

Siser, i'm sure you've seen this, but in case you haven't, here.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
Dr_Sister
#18 Posted : 4/20/2011 4:22:12 PM

Synaptic cleft explorer

Chemical expert | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, MarketingSenior Member | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, Marketing

Posts: 299
Joined: 10-Dec-2010
Last visit: 13-Feb-2014
Location: good question
benzyme wrote:

Siser, i'm sure you've seen this, but in case you haven't, here.



No, I'd overlooked that Benzyme. Thank you.
 
Dr_Sister
#19 Posted : 5/30/2011 3:55:47 PM

Synaptic cleft explorer

Chemical expert | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, MarketingSenior Member | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, Marketing

Posts: 299
Joined: 10-Dec-2010
Last visit: 13-Feb-2014
Location: good question
Well, after several attempts Sister has something that is getting close, but no cigar yet.

Ehrlich's reagent is working fine, although it needs some heat to develop fully.

Initially sister was getting huge blobs as results, but after diluting the product and switching micropipettes Sister is now getting thin streaks, very consistently. When product is diluted even further she gets no results, so Sister thinks overloading the sample is not the problem.

Would adding some NH4OH help with this streaking problem? The anticipated product being largely amines.

Also the rf for the top of the streak (also darkest spot in the streak) is like rf 0.9, so sister is wondering if a less polar eluent might be called for? She has been using EtAcO/MeoH - 3:1

Has anyone else encountered this issue? And if so, how did they work around?

Thanks
Sister
 
Dr_Sister
#20 Posted : 5/30/2011 4:03:21 PM

Synaptic cleft explorer

Chemical expert | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, MarketingSenior Member | Skills: Chemistry, Horticulture, Yoga, Meditation, Graphic/web design, Marketing

Posts: 299
Joined: 10-Dec-2010
Last visit: 13-Feb-2014
Location: good question
One other question about Ehrlich's reagent. Sister has been trying it on other compounds as well and notices slightly different colours depending on what amine is spotted. The colours range from teal to blue to purple. Is there any way the colour of the spot can be used to determine some of the characteristics of the compound being spotted?

For instance would a primary or secondary amine give a different colour result than a tertiary or saturated amine? Would a beta-carboline give a different colour from an uncyclized amine?

This is a long shot, but is anyone aware of the existence of a colour chart to interpret these results? Any refernces would be greatly appreciated.

Thanks again
Sister
 
12NEXT
 
Users browsing this forum
Guest

DMT-Nexus theme created by The Traveler
This page was generated in 0.038 seconds.