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tievine (ipomoea cordatotriloba) Options
 
benzyme
#1 Posted : 6/19/2022 4:48:47 PM

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I generated some data, which to my knowledge, is the first ms data (or any analytical data) for these particular variety of seeds. I compared against I.tricolor var. "heavenly blue". The masses were within two mass units of lysergol standard.
The heavenly blue seeds were considerably larger, about triple the size. All samples were ground with mortar and pestle, 710 mg transferred to 15 mL conicles, with 7.1 mL methanol, and vortexed for a min, filtered through 0.22 um syringe filters to 2 mL autosampler vials.

I've searched everywhere for data on I.cordatotriloba, it's not even referenced in The Encyclopedia of Psychoactive Plants.

Will upload the MS data, and perform FLD and CAD analysis.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 

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_Trip_
#2 Posted : 6/20/2022 2:15:45 AM

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According to this study: https://www.nature.com/a...cles/s42003-021-02870-z or atleast the attached diagrams they do not contain ergot alkaloids. I would not be surprised if such a large study contains inaccuracies.

But very interested to see your results benzyme.
Disclaimer: All my posts are of total fiction.



 
benzyme
#3 Posted : 6/20/2022 2:55:54 PM

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ahh nice...such a recent study too. Thanks. Big grin

this is preliminary data, and will be an ongoing study.
I overloaded the detector with lysergol standard (oops), it really sticks to the column. No cross-contam was performed, all samples were dissolved in HPLC-grade methanol.
benzyme attached the following image(s):
tievine extract.jpg (169kb) downloaded 77 time(s).
heavenly blue extract.jpg (162kb) downloaded 76 time(s).
lysergol std.jpg (169kb) downloaded 74 time(s).
IMG_1081.jpg (278kb) downloaded 73 time(s).
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
benzyme
#4 Posted : 6/20/2022 3:16:20 PM

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I ground up the tievine seeds first, added methanol, and transferred to an autosampler vial. The MG seeds were treated the same, and used as a suspected known. Lysergol was a dark beige powder, and ~1mg was added straight to an autosampler vial with methanol. All samples were filtered with new 0.22 um ptfe syringe filters.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
_Trip_
#5 Posted : 6/21/2022 1:27:32 AM

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Those results look very promising benzyme.
So ipomoea cordatotriloba appears active lookimg at the MS data. In addition, it's a more cold hardy ipomoea. If it has decent percentage of ergine alkaloids this could be a better species to grow for those in colder climates.
Disclaimer: All my posts are of total fiction.



 
ava69
#6 Posted : 6/22/2022 9:24:37 PM

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Benzyme said:
Quote:
I generated some data, which to my knowledge, is the first ms data (or any analytical data) for these particular variety of seeds. I compared against I.tricolor var. "heavenly blue". The masses were within two mass units of lysergol standard.
The heavenly blue seeds were considerably larger, about triple the size. All samples were ground with mortar and pestle, 710 mg transferred to 15 mL conicles, with 7.1 mL methanol, and vortexed for a min, filtered through 0.22 um syringe filters to 2 mL autosampler vials.

I've searched everywhere for data on I.cordatotriloba, it's not even referenced in The Encyclopedia of Psychoactive Plants.

Will upload the MS data, and perform FLD and CAD analysis.

Very cool Benzyme and _Trip_
I also own the Encyclopedia of psychoactive plants by Ratsch, have a whole bookshelf of entheogenic books...love that one for sure, read it twice.
 
benzyme
#7 Posted : 6/23/2022 1:51:25 PM

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thanks Big grin
I also have that one on my shelf. It really is an indespensible read, incredibly useful.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
_Trip_
#8 Posted : 6/24/2022 10:02:51 AM

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Benzyme, will you be able to calculate a rough percentage?
Disclaimer: All my posts are of total fiction.



 
benzyme
#9 Posted : 6/24/2022 3:21:34 PM

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yes, with CAD detection, I could run a comprehensive purity profile (that detection method shows everything). FLD, to some extent (in the UV domain), would as well.

I ran a 3D-excitation scan, from 260 nm to 330, 450 nm emission. The results were curious. The tievine main component eluted earlier than the lysergol standard, and had peak excitations around 292 nm, 304, and 320.. I need to run an erlich's test to screen for indolic compounds; if it gives a purple hue, it may contain some clavine compound.
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
endlessness
#10 Posted : 6/25/2022 12:47:01 PM

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Thanks for sharing your ongoing experiment, please do let us know the final results!

Are we seeing two co-eluting substances in the tievine extract there?

What about the MG extract, what is the minor peak?
 
 
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