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Biotransformation of MiPT to 4-HO-MiPT Options
 
benzyme
#1 Posted : 5/23/2020 10:59:28 PM

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after much contemplation, I finally got around to prepping PFtek jars with 15 mM MiPT (made from tryptamine, derived from tryptophan) as an experimental group, and a control group without MiPT. Characterization will be by HPLC-UV-MS.

https://www.reddit.com/r...ansformation_of_mipt_to/
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dragonrider
#2 Posted : 5/23/2020 11:20:20 PM

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Would this work as well with organisms that produce bufotenin?
 
downwardsfromzero
#3 Posted : 5/23/2020 11:26:24 PM

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dragonrider wrote:
Would this work as well with organisms that produce bufotenin?

Maybe not. The 5-hydroxylation of tryptophan occurs before decarboxylation in that pathway, I think. Not much is known about the isopropyl analogue of bufotenine.
Ora, lege, lege, lege, relege et labora

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benzyme
#4 Posted : 5/25/2020 3:33:16 PM

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Big grin

Thanks for the support. It's been discussed before, I'm just fortunate of having the means of characterizing the end products. LCMS determination of psilocin/psilocybin in mushrooms is something I'm no stranger to, and I have spectra for the MiPT.
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benzyme
#5 Posted : 8/15/2020 7:27:56 PM

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welp, those spores were nonviable. I just innoc'd some jars with a new syringe (most vendors have opted for syringes vs. prints), with 20 mM MiPT, and a control jar.

I also ordered a syringe, and a print, of c.cyanescens. That species may particularly have stronger expression of PsiH, a gene which encodes for tryptamine-4-monooxygenase (a CYP450 enzyme, nonspecific oxidoreductase). Said gene was only recently characterized, by Fricke et. al, 2017.
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"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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benzyme
#6 Posted : 12/6/2020 5:38:02 PM

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Confused
benzyme attached the following image(s):
control.jpg (343kb) downloaded 54 time(s).
experimental.JPG (204kb) downloaded 52 time(s).
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"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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RoundAbout
#7 Posted : 12/6/2020 10:00:03 PM

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I guess the main peak in the control is a fragment from the alpha cleavage of psilocybin, but what are the main peaks in the experimental? Out of curiousity...
 
benzyme
#8 Posted : 12/7/2020 12:08:27 AM

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RoundAbout wrote:
I guess the main peak in the control is a fragment from the alpha cleavage of psilocybin, but what are the main peaks in the experimental? Out of curiousity...


your guess is as good as mine. I didn't run any standards
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
XMR 46JqfBqi7JNM1W5rXXZ1FF1CBKKLoGEVBRya9Cn3RJmPbQhT4GeZpVKWbwaVe4vUMveKAzAiA4j8xgUi29TpKXpm3whx5MW
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