I have with Psilohuasca, although i'm sure it's possible with 4-ACO on it's own too. 4-ACO definitely turns into Psilocin, especially noticeable with the Harmalas in the mix, 4-ACO on it's own does feel a bit different though compared to mushrooms, i'm thinking because 4-ACO is active on it's own before being metabolized into Psilocin, and because the mushroom contains other compounds which synergize with and alter the Psilocin, but i've taken both mushrooms, and 4-ACO, with Harmalas, and the resulting Psilohuascas were nearly identical ime, same experience, same effects, same lengthening of the duration, the Psilocin is VERY noticeable, and sometimes things would feel DMT-like, and sometimes it'd feel mushroom-like, in fact anytime i take 4-ACO with Rue, i get imagery of mushrooms, and a very very very mushroomy vibe, like some straight up mushroom extract or something, it felt more mushroomy than mushrooms have on their own, also more visual ime, especially compared to oral DMT which i don't typically see closed eyed visuals from oral DMT but i seem to with Psilocin. Also seems to take me to the same states as Aya with oral DMT but in a gentler/more relaxed fashion compared to DMT's serious Adrenergic edge, so Psilocin with Harmalas is like oral DMT with Harmalas but a little different, and softer compared to DMT's "holy shit" harsh intensity.