In the mass spec, it was psilocin that moved into Xylene in the peak that benzyme found.
While the resulting powder after FASA seems stable, yields are low.
Psilocybin movement onto Xylene was negligable as I remember.
Other solvents you can easily get over the counter used for DMT didn't work, with the possible exception of limonene. That did form clouds, but was never tested.
If you really want to do this without Xylene I would try limonene and first convert everything to psilocin. Using vitamin C may help yield, but not sure. Also work in the dark and quickly.
What I would do after many tests if you really want an "A/B" like process,
- 10g of mushrooms in water, add VitC to pH4 (a few grams), extract at 70C for one our
- Filter and boil for just a few minutes (boiling will help reduce emulsions later from my experience)
- Add 30% ammonium sulphate, a bunch of stuff will crash out, you can filter it
- Adjust pH to about 8.5 (near the pI but not too basic to destroy all the psilocin hopefully - you can try lower pH too maybe like 7, it's about trying to find a mild alkaline place where psilo moves into Limonene but still doesn't degrade significantly while in water)
- Limonene pull
- Fumarate crash/decant
- Fumarate wash in dry acetone
You may get something with this. If you can use Xylene, that has shown to give some psilocin in MS and bioassay, but again yields were low.
Instead of all this, I would reccomend buying cation resin (used for filtering hard water). Then buy tryptophan. Get a cheap two foot tube and make a column. Read the two psilo isolation papers using cation resin from 2010/11. Play around with the tryptophan until you can get it on/off the column. Then copy that process on mushroom extract. Check out the cation resin experiments we are doing in the forum along these lines. I can put the links here if you have trouble finding them.