Vinegar, or any acid really, ruins the taste of the brew and is not advised. You only need an acid if you are trying to brew really quickly. 2x 50 sounds sufficient, but maybe next time try 3x 30. Another wash of water may pull more alkaloids it may not, experiment.
Your increase in dose size I think is due to your decanting steps. Because you went to such lengths to have a clear brew, you probably also left some actives behind. This would be my guess.