Is your intention just to trip harder, or do you have a specific purpose for wanting to work with brugs?
Also, are you familiar with how tropanes accumulate in the body? To be ultra safe, I would space each dose two weeks apart at minimum.
I have been contemplating working with Brunfelsia Grandiflorum for months. It contains tropanes, though in far smaller quantities than Brugmansia. Can also cause respiratory depression and make you foam at the mouth, so not to be trifled with.
I think a long, slow dieta with a large and consistent batch of plant matter is the only close-to-surefire way of not killing yourself or ending up in a psych ward (but again, no guarantees).
It's important with any kind of baneful plant to have a method of standardizing the alkaloid content. I know Datura can vary from leaf to leaf, or seed to seed, so traditionally the sanest method has been to take a large amount of plant material and make a salve. Topical is also safer with tropanes, but I'm not sure how this would work with Ayahuasca.
If you are purely after the tropanes, topical application in conjunction with vanilla Aya might be a method of harm reduction to consider. No idea about what an ideal timing of administration would be.
I am interested in the coumarins in Brunfelsia, so oral is the only way for me to go.
If I do it, I would stew up a massive batch of plant matter and freeze most of it. I would start with literally ONE DROP with a brew, and wait a minimum of two weeks before increasing my dose and trying again.
I'm also not interested in going as far as I possibly can with Brunfelsia - just a minimum effective dose to make contact with the spirit and see if he can help me with some CNS issues.
Realistically, we have our entire lives ahead of us - it is much better to approach these plants at a snail's pace and learn them intimately. IMHO the potential for harm to just dive in, even with a few seeds, isn't worth it. There are too many variables to just down a handful of seeds. Because the stakes are literally cardiac integrity and lesions on the brain, better to explore inch by inch.
There are some really sad stories on Bluelight and Reddit.
There are some intriguing ones, too, but ultimately - what is your purpose? I would make sure you are 100% firm in your business with these spirits, and approach with absurd reverence.
I think Peter Gorman's Ayahuasca In My Blood is also worth reading, if you haven't, for his advice about machismo and being (for lack of a better term) shit tested by the spirits. You need to be ready to stand at the gates of hell, scream your name and your purpose. If you aren't that confident, don't pull the trigger.
Some things will come easy, some will be a test